P2X1 antibody

Cat. No. GTX16925

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, FACS, LCI
Reactivity	Human, Mouse, Rat
Package	50 μl ($639)

See all P2X1 products

Datasheet
Document

View product citations for antibody GTX16925 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
LCI	Assay dependent

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 (Extracellular loop) of human P2X1 receptor (Accession : P51575).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purinergic receptor P2X 1 , P2X1

Cellular Localization

Membrane; Multi-pass membrane protein

Background

The protein encoded by this gene belongs to the P2X family of G-protein-coupled receptors. These proteins can form homo-and heterotimers and function as ATP-gated ion channels and mediate rapid and selective permeability to cations. This protein is primarily localized to smooth muscle where binds ATP and mediates synaptic transmission between neurons and from neurons to smooth muscle and may being responsible for sympathetic vasoconstriction in small arteries, arterioles and vas deferens. Mouse studies suggest that this receptor is essential for normal male reproductive function. This protein may also be involved in promoting apoptosis. [provided by RefSeq, Jun 2013]

Database

Gene ID: 5023 P2RX1
UniProt: P51575 P2RX1

Research Area

DATA IMAGES

GTX16925 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX16925 P2X1 antibody.
Panel A : Most of P2RX1 (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples).
Panel B : DAPI is used as the counterstain (blue).
Panel C : Merge of A and B.

GTX16925 LCI Image

Live cell imaging analysis of SH-SY5Y cells using GTX16925 P2X1 antibody.
Panel A : Primary antibody (red)
Panel B : DAPI for nuclear staining (blue)
Panel C : Merged images of Panel A and B
Dilution : 1:50

GTX16925 FACS Image

FACS analysis of MEG-O1 cells using GTX16925 P2X1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Antibody amount : 10 μg antibody / 1 x 10⁶ cells

GTX16925 WB Image

WB analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates using GTX16925 P2X1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 639

Qty