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PABPC4 antibody

Anti-PABPC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33380
Anti-PABPC4 antibody used in Western Blot (WB). GTX33380
Anti-PABPC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33380
Anti-PABPC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33380

Cat. No. GTX33380

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PABPC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC4 (NP_003810.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(A) binding protein cytoplasmic 4 , APP-1 , APP1 , PABP4 , iPABP

Cellular Localization

Cytoplasm

Background

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-PABPC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33380

GTX33380 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33380 PABPC4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PABPC4 antibody used in Western Blot (WB). GTX33380

GTX33380 WB Image

WB analysis of various sample lysates using GTX33380 PABPC4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PABPC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33380

GTX33380 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33380 PABPC4 antibody.
Dilution : 1:100

Anti-PABPC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33380

GTX33380 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX33380 PABPC4 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399