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PAI-1 antibody, Internal

Anti-PAI-1 antibody, Internal used in Western Blot (WB). GTX89568
Anti-PAI-1 antibody, Internal used in Western Blot (WB). GTX89568
Anti-PAI-1 antibody, Internal used in ELISA (ELISA). GTX89568
Anti-PAI-1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89568

Cat. No. GTX89568

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($399)
See all PAI-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 4-6μg/ml
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

IHC-P
Human Placenta shows strong staining of cytotrophoblasts.
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89568-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family E member 1 , PAI , PAI-1 , PAI1 , PLANH1

Cellular Localization

Secreted

Background

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PAI-1 antibody, Internal used in Western Blot (WB). GTX89568

GTX89568 WB Image

WB analysis of A549 (A), HepG2 (B) and HeLa (C) lysates using GTX89568 PAI-1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PAI-1 antibody, Internal used in Western Blot (WB). GTX89568

GTX89568 WB Image

WB analysis of human liver lysate using GTX89568 PAI-1 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PAI-1 antibody, Internal used in ELISA (ELISA). GTX89568

GTX89568 ELISA Image

ELISA analysis of PAI-1 protein using GTX89568 PAI-1 antibody, Internal.
Capture : GTX100550 (2.5μg/ml)
Detection : GTX89568 (1.5μg/ml)

Anti-PAI-1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89568

GTX89568 IHC-P Image

IHC-P analysis of human placenta using GTX89568 PAI-1 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399