GeneTex

  • Country / Location Selection

United States (US)

PAK (phospho Ser141) antibody

Orthogonal
Anti-PAK (phospho Ser141) antibody used in Western Blot (WB). GTX25247
Anti-PAK (phospho Ser141) antibody used in Western Blot (WB). GTX25247

Cat. No. GTX25247

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat, Bovine
Package
50 μl ($399)
See all PAK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide derived from a region of human PAK2 containing serine 141. The sequence is conserved in rat and rabbit.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380488

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

p21 (RAC1) activated kinase 1 , p21 (RAC1) activated kinase 2 , p21 (RAC1) activated kinase 3 , IDDMSSD , PAKalpha , PAK65 , PAKgamma , ARA , MRX30 , MRX47 , OPHN3 , PAK-3 , PAK3beta , bPAK , beta-PAK

Cellular Localization

Cytoplasm,Cell membrane,nucleoplasm

Background

P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Database

Research Area

DATA IMAGES

Anti-PAK (phospho Ser141) antibody used in Western Blot (WB). GTX25247

GTX25247 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of SH-SY5Y (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), HeLa (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), K562 (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX25247 PAK2 (phospho Ser141) antibody.
Dilution : 1:500

Anti-PAK (phospho Ser141) antibody used in Western Blot (WB). GTX25247

GTX25247 WB Image

Orthogonal

WB analysis of samples using GTX25247 PAK2 (phospho Ser141) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Chen MJ et al. Sci Rep 2016; 6 : 34933 PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PAK (phospho Ser141) antibody (GTX25247). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399