PAK1 (phospho Thr212) antibody

Cat. No. GTX78974

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX78974 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-Tp-P-T).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11168231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PAK1,PAKalpha,p21 (RAC1) activated kinase 1

Cellular Localization

Cytoplasm,Cell junction,Focal adhesion,Cell membrane,Cell projection,Ruffle membrane,Invadopodium

Background

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Database

Gene ID: 5058 PAK1
UniProt: Q13153 PAK1

Research Area

DATA IMAGES

GTX78974 WB Image

WB analysis of 293 cells untreated or treated with forskolin (40μM, 30min) lysates using GTX78974 PAK1 (phospho Thr212) antibody (Lane 3 and 4) and PAK1 antibody (Lane 1 and 2).

GTX78974 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78974 PAK1 (phospho Thr212) antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty