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PARC antibody

Anti-PARC antibody used in Western Blot (WB). GTX85085
Anti-PARC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85085
Anti-PARC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85085

Cat. No. GTX85085

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PARC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

281 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC.The immunogen is located within the last 50 amino acids of PARC.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cullin 9 , H7AP1 , PARC

Cellular Localization

Cytoplasm

Background

The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.

Database

Research Area

DATA IMAGES

Anti-PARC antibody used in Western Blot (WB). GTX85085

GTX85085 WB Image

WB analysis of Daudi cell lysate using GTX85085 PARC antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PARC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85085

GTX85085 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85085 PARC antibody.
Working concentration : 2 μg/ml

Anti-PARC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85085

GTX85085 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85085 PARC antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399