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PARD3 antibody

Anti-PARD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00653
Anti-PARD3 antibody used in Western Blot (WB). GTX00653

Cat. No. GTX00653

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PARD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

151 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PARD3, corresponding to a region within C-terminal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ASIP , Baz , PAR3 , PAR3alpha , PARD3 , PARD3A , PPP1R118 , SE25L16 , SE25LT1 , SE25T2 , par3 family cell polarity regulator

Cellular Localization

Cytoplasm,Endomembrane system,Cell junction,Tight junction,Adherens junction,Cell membrane,Cell cortex,Cytoskeleton

Background

This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PARD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00653

GTX00653 ICC/IF Image

ICC/IF analysis of PFA-fixed COLO205 cells using GTX00653 PARD3 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-PARD3 antibody used in Western Blot (WB). GTX00653

GTX00653 WB Image

WB analysis of mouse brain tissue lysate using GTX00653 PARD3 antibody. The lane on the right was treated with blocking peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399