APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 5-10μg/ml |
|
Note :
IHC-P
Human Pancreas shows strong cytoplasmic staining in rare cells of intralobular ducts.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX26022-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1; NP_001032358.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
par-6 family cell polarity regulator alpha , PAR-6A , PAR6 , PAR6C , PAR6alpha , TAX40 , TIP-40
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX26022 IHC-P Image
IHC-P analysis of human pancreas using GTX26022 PARD6A antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10μg/ml
|
|
|
GTX26022 IHC-P Image
IHC-P analysis of human heart using GTX26022 PARD6A antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
REFERENCE
There are currently no references for PARD6A antibody, C-term (GTX26022). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PARD6A antibody, C-term (GTX26022). Be the first to share your experience with this product.
