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PARP14 antibody

Anti-PARP14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131920
Anti-PARP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX131920
Anti-PARP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX131920
Anti-PARP14 antibody used in Immunoprecipitation (IP). GTX131920
Anti-PARP14 antibody used in Western Blot (WB). GTX131920
Anti-PARP14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131920

Cat. No. GTX131920

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PARP14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

203 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PARP14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886538

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(ADP-ribose) polymerase family member 14 , ARTD8 , BAL2 , PARP-14 , pART8

Cellular Localization

Nucleus , Cytoplasm

Background

Poly(ADP-ribosyl)ation is an immediate DNA damage-dependent posttranslational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP14 belongs to the superfamily of enzymes that perform this modification (Ame et al., 2004 [PubMed 15273990]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PARP14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131920

GTX131920 ICC/IF Image

PARP14 antibody detects PARP14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP14 stained by PARP14 antibody (GTX131920) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PARP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX131920

GTX131920 IHC-P Image

PARP14 antibody detects PARP14 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human spleen.
PARP14 stained by PARP14 antibody (GTX131920) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP14 antibody used in IHC (Paraffin sections) (IHC-P). GTX131920

GTX131920 IHC-P Image

PARP14 antibody detects PARP14 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human spleen.
PARP14 stained by PARP14 antibody (GTX131920) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PARP14 antibody used in Immunoprecipitation (IP). GTX131920

GTX131920 IP Image

Immunoprecipitation of PARP14 protein from SK-N-SH whole cell extracts using 5 μg of PARP14 antibody (GTX131920).
Western blot analysis was performed using PARP14 antibody (GTX131920).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PARP14 antibody used in Western Blot (WB). GTX131920

GTX131920 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP14 antibody (GTX131920) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PARP14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131920

GTX131920 ICC/IF Image

PARP14 antibody detects PARP14 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PARP14 stained by PARP14 antibody (GTX131920) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169