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PAX3 antibody [GT1210]

Orthogonal
Anti-PAX3 antibody [GT1210] used in Western Blot (WB). GTX634390
Anti-PAX3 antibody [GT1210] used in IHC (Paraffin sections) (IHC-P). GTX634390

Cat. No. GTX634390

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1210

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PAX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PAX3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888444

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 3 , CDHS , HUP2 , WS1 , WS3

Cellular Localization

Nucleus

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAX3 antibody [GT1210] used in Western Blot (WB). GTX634390

GTX634390 WB Image

Orthogonal

U87-MG whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [GT1210] (GTX634390) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PAX3 antibody [GT1210] used in IHC (Paraffin sections) (IHC-P). GTX634390

GTX634390 IHC-P Image

PAX3 antibody [GT1210] detects PAX3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
PAX3 stained by PAX3 antibody [GT1210] (GTX634390) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169