APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AN , AN2 , D11S812E , FVH1 , MGDA , PAX6 , WAGR , paired box 6
Cellular Localization
Nucleus
Background
This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain. [provided by RefSeq, Mar 2019]
Database
Research Area
DATA IMAGES
![Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956](/templates/default/js/lightbox/images/loading.gif "Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956")
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GTX34956 IHC-P Image
IHC-P analysis of human gastric carcinoma tissue using GTX34956 PAX6 antibody [PAX6/1166].
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REFERENCE
There are currently no references for PAX6 antibody [PAX6/1166] (GTX34956). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAX6 antibody [PAX6/1166] (GTX34956). Be the first to share your experience with this product.
![Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956](/upload/website/prouct_img/small/GTX34956/GTX34956_20200115_IHC-P_593_w_23060801_651.webp "Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956")
![Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956](/upload/website/prouct_img/normal/GTX34956/GTX34956_20200115_IHC-P_593_w_23060801_651.webp "Anti-PAX6 antibody [PAX6/1166] used in IHC (Paraffin sections) (IHC-P). GTX34956")