APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , A431 , HeLa
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PBR. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886302
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
translocator protein , BPBS , BZRP , DBI , IBP , MBR , PBR , PBS , PKBS , PTBR , mDRC , pk18
Background
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]
Database
Research Area
DATA IMAGES
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GTX130550 WB Image
Wild-type (WT) and PBR knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody (GTX130550) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130550 WB Image
PBR antibody detects PBR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody (GTX130550) diluted at a dilution of 1:1000.
MCF7 is known to express little to none of PBR. Reference: Cancer Res. 1999 Feb 15;59(4):831-42.
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GTX130550 IP Image
Immunoprecipitation of PBR protein from A431 whole cell extracts using 5 μg of PBR antibody (GTX130550).
Western blot analysis was performed using PBR antibody (GTX130550).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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REFERENCE
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REVIEW
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