PC-12 nuclear extract (Forskolin stimulated) was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. Forskolin is a diterpene compound that activates adenylyl cyclase, thus elevating intracellular levels of cAMP.
20 mM HEPES (pH 7.5), 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na₂MoO₄, 1 mM NaVO₃, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT, and protease inhibitors
Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
2.50 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.