PCAF antibody [C3], C-term

Name: PCAF antibody [C3], C-term
Brand: GeneTex
SKU: GTX109666
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

Cat. No. GTX109666

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX109666 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , A375 , HeLa nuclear extract

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PCAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950609

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine acetyltransferase 2B , CAF , P/CAF , PCAF

Cellular Localization

Nucleus

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq]

Database

Gene ID: 8850 KAT2B
UniProt: Q92831 KAT2B

Research Area

DATA IMAGES

GTX109666 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX109666 diluted at 1:1000

GTX109666 WB Image

PCAF antibody [C3], C-term detects PCAF protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PCAF antibody [C3], C-term (GTX109666) dilution: 1:1000

GTX109666 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX109666) diluted at 1:1000.

REFERENCE

Application Reference

Wang SA et al. Sci Rep 2020; 10 (1) : 20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.

Application : WB

Reactivity : Human

Sengupta S et al. DNA Repair (Amst) 2018; 66-67 : 1-10 Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.

Cazzalini O et al. Nucleic Acids Res 2014; CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.

Application : WB

Reactivity : Human

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