APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| 5-10 μg |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat, MDCK
Product Note
PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily. It interacts with the p300 and CBP (CREB-binding protein) transcriptional coactivators and may be found in multi-subunit protein complexes formed from more than 20 polypeptides ranging from 10 to 400 kDa.PCAF shows extensive sequence similarity to human and mouse GCN5 HAT. Human PCAF acetylates all four core-histones. It also acetylates nonhistone proteins such as p53, MyoD, TFIIE-β, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF contributes to transcriptional activation by modifying chromatin and transcriptional factors. It is required in many cell growth and development processes such as myogenesis, and in nuclear receptor mediated or growth factor-signaled cell activation.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken. synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lysine acetyltransferase 2B , CAF , P/CAF , PCAF
Cellular Localization
Nucleus
Background
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
Database
Research Area
DATA IMAGES
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GTX12188 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.
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GTX12188 WB Image
WB analysis of Y79 cell lysate using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)
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GTX12188 WB Image
WB analysis of (Lane 1-2) HeLa, (Lane 3-4) HepG2, (Lane 5-6) Jurkat cell lysate using GTX12188 PCAF antibody at 2 μg/mL.
Lane 1,3,5 : Antibody 2 μg/mL
Lane 2,4,6 : Antibody 2 μg/mL + PCAF immunizing peptide
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GTX12188 WB Image
WB analysis of human PCAF recombinant protein using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Negative control (without antibody)
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GTX12188 IP Image
IP analysis of Jurkat cell lysate using GTX12188 PCAF antibody.
Lane 1 : 5 μg/mL antibody
Lane 2 : Negative control: without antibody
Lane 3 : 10 μg/mL antibody
Lane 4 : Negative control: without cell extract
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REFERENCE
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REVIEW
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