APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
5-10μg/ml |
Note :
IHC-P
Human Testis shows strong staining of cytoplasm in select spermatocytes.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89456-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NAWKQNLNKVKN, from the C Terminus of the protein sequence according to NP_006351.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 3 subunit M , B5 , GA17 , PCID1 , TANGO7 , hfl-B5
Cellular Localization
Cytoplasm
Background
This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome. [provided by RefSeq, Dec 2011]
Database
Research Area
DATA IMAGES
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GTX89456 WB Image
WB analysis of A549 lysate using GTX89456 PCID1 antibody, C-term. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
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GTX89456 IHC-P Image
IHC-P analysis of human testis using GTX89456 PCID1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
There are currently no references for PCID1 antibody, C-term (GTX89456). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PCID1 antibody, C-term (GTX89456). Be the first to share your experience with this product.