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PCNA antibody [339]

Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298
Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298
Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298
Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298
Anti-PCNA antibody [339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70298

Cat. No. GTX70298

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 339

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PCNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant PCNA interdomain connector loop protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_377591

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298

GTX70298 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: NIH-3T3
C: PC-12
12% SDS PAGE
GTX70298 diluted at 1:1000

Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298

GTX70298 WB Image

Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298

GTX70298 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
12% SDS PAGE
GTX70298 diluted at 1:1000

Anti-PCNA antibody [339] used in Western Blot (WB). GTX70298

GTX70298 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
12% SDS PAGE
GTX70298 diluted at 1:1000

Anti-PCNA antibody [339] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70298

GTX70298 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using (GTX70298) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations