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PCNA antibody [PC10]

Orthogonal
Anti-PCNA antibody [PC10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20029
Anti-PCNA antibody [PC10] used in Western Blot (WB). GTX20029
Anti-PCNA antibody [PC10] used in Flow cytometry (FACS). GTX20029
Anti-PCNA antibody [PC10] used in IHC (Paraffin sections) (IHC-P). GTX20029

Cat. No. GTX20029

Reference 24

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PC10

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Fish
Package
100 μg ($399)
See all PCNA products

APPLICATION

Application Note

Flow Cyt: Use at an assay dependent dilution. IHC-P: 1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol IHC-Fr: Use at an assay dependent dilution. Fix in 4% buffered formalin at room temperature for 2 min then fix in ethanol for 10 minutes. IP: Use at an assay dependent dilution WB: 1:5,000, Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

29 kDa. ( Note )

Positive Control

WB: DT40 B lymphoma cell lysate, 293 cell lysate

Product Note

Specific for PCNA p36 protein expressed at high levels in proliferating cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_369854

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [PC10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20029

GTX20029 ICC/IF Image

Human Tonsil stained with Anti-CD3, T Cell at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen (GTX20029) at a 1:500 dilution (green). Counterstained with DAPI.

Anti-PCNA antibody [PC10] used in Western Blot (WB). GTX20029

GTX20029 WB Image

Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA (GTX20029)

Anti-PCNA antibody [PC10] used in Flow cytometry (FACS). GTX20029

GTX20029 FACS Image

Staining of KM-H2 cells with MOUSE ANTI PCNA:FITC (GTX75144)following permeabilisation

Anti-PCNA antibody [PC10] used in IHC (Paraffin sections) (IHC-P). GTX20029

GTX20029 IHC-P Image

Orthogonal

The data was published in the journal J Clin Med in 2019. PMID: 30871022

REFERENCE

Application Reference

Yeom M et al. Chem Res Toxicol 2020; 33 (8) : 2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Application : WB
Reactivity : Human
Bryche B et al. Neural Regen Res 2020; 15 (2) : 352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Application : IHC-Fr
Reactivity : Rat
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Application : IHC-P
Reactivity : Human
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
Application : IHC-Fr
Reactivity : Mouse

Clone Reference

?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
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REVIEW

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Application Reference
Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Application : WB
Reactivity : Human
Bryche B et al. Neural Regen Res 2020; 15 (2):352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Application : IHC-Fr
Reactivity : Rat
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Application : IHC-P
Reactivity : Human
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
Application : IHC-Fr
Reactivity : Mouse
Chauvigne F et al. PLoS One 2017; 12 (3):e0174387 toward developing recombinant *-based hormone therapies for increasing fertility in the flatfish senegalese sole.
Reactivity : Fish
Chauvigne F et al. Comp Biochem Physiol A Mol Integr Physiol 2017; 206:36-46 Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis.
Application : IHC-P
Reactivity : Fish
Riviere S et al. Sci Rep 2016; 6:34011 High Fructose Diet inducing diabetes rapidly impacts olfactory epithelium and behavior in mice.
Francois A et al. Sci Rep 2016; 6:24687 Olfactory epithelium changes in germfree mice.
Application : IHC-Fr
Reactivity : Mouse
Gu LH et al. Cell Mol Immunol 2015; 12 (4):444-55 Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats.
Application : IHC-Fr
Reactivity : Rat
Boj M et al. PLoS One 2015; 10 (11):e0142512 *-activated androgen-dependent and independent pathways regulate aquaporin expression during teleost (sparus aurata) spermatogenesis.
Application : ICC/IF
Reactivity : Fish
Aurelien Raynaud et al. Behavioural Brain Research 2015; 15 (291):36-45 Chronic variable stress exposure in male Wistar rats affects the first step of olfactory detection
Application : WB
Reactivity : Rat
Liu Y et al. Cytotechnology 2014; Lupeol induces apoptosis and inhibits invasion in gallbladder carcinoma GBC-SD cells by suppression of EGFR/MMP-9 signaling pathway.
Turpin W et al. PLoS One 2013; 8 (4):e57711 Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
Application : IHC-P
Reactivity : Rat
Tomas J et al. FASEB J 2013; 27 (2):645-55 Primocolonization is associated with colonic epithelial maturation during conventionalization.
Application : IHC
Reactivity : Rat
Rul F et al. J Biol Chem 2011; 286 (12):10288-96 Impact of the metabolic activity of Streptococcus thermophilus on the colon epithelium of gnotobiotic rats.
Application : IHC
Cherbuy C et al. Am J Physiol Gastrointest Liver Physiol 2010; 299 (2):G348-57 Microbiota matures colonic epithelium through a coordinated induction of cell cycle-related proteins in gnotobiotic rat.
Application : IHC
Reactivity : Rat
Ji RC et al. Microvasc Res 2010; 80 (2):195-201 Multiple expressions of lymphatic markers and morphological evolution of newly formed lymphatics in lymphangioma and lymph node lymphangiogenesis.
Application : IHC
Reactivity : Mouse
Guo Z et al. Nat Chem Biol 2010; 6 (10):766-73 Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Application : ICC/IF
Reactivity : Human
Kumar R et al. Clin Cancer Res 2009; 15 (12):4123-30 extranuclear coactivator signaling confers insensitivity to *.
Application : IHC
Reactivity : Mouse
Krasna L et al. Breast Cancer Res Treat 2002; 71 (3):219-35 Large expansion of morphologically heterogeneous mammary epithelial cells, including the luminal phenotype, from human breast tumours.
Application : IHC-P
Reactivity : Human
Clone Reference
?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
Ozaydin T et al. Microsc Res Tech 2016; Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages.
Wen Q et al. PLoS One 2012; 7 (5):e37503 HGF-transgenic MSCs can improve the effects of tissue self-repair in a rabbit model of traumatic osteonecrosis of the femoral head.
Application : IHC-P
Reactivity : Rabbit
Yasemin O et al. Journal of Animal and Veterinary Advances. 2009; 8(9):1694-701 Comparative Skin Histology of the White New Zealand and Angora Rabbits: Histometrical and Immunohistochemical Evaluations.
Application : IHC
Package List Price ($)
$ 399
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