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PCNA antibody [PCNA/694]

Anti-PCNA antibody [PCNA/694] used in Flow cytometry (FACS). GTX34964
Anti-PCNA antibody [PCNA/694] used in IHC (Paraffin sections) (IHC-P). GTX34964
Anti-PCNA antibody [PCNA/694] used in Western Blot (WB). GTX34964

Cat. No. GTX34964

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PCNA/694

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all PCNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length human PCNA protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATLD2 , PCNA , proliferating cell nuclear antigen

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [PCNA/694] used in Flow cytometry (FACS). GTX34964

GTX34964 FACS Image

FACS analysis of PFA-fixed HeLa cells using GTX34964 PCNA antibody [PCNA/694].
Blue : Primary antibody
Red : Isotype control

Anti-PCNA antibody [PCNA/694] used in IHC (Paraffin sections) (IHC-P). GTX34964

GTX34964 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34964 PCNA antibody [PCNA/694].

Anti-PCNA antibody [PCNA/694] used in Western Blot (WB). GTX34964

GTX34964 WB Image

WB analysis of HepG2 cell lysate using GTX34964 PCNA antibody [PCNA/694].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529