PD-L1 antibody [29E.2A3] (PerCP-Cy5.5)

Cat. No. GTX01495-11

Host	Mouse
Clonality	Monoclonal
Clone Name	29E.2A3
Isotype	IgG2b
Application	FACS
Reactivity	Human
Package	100 test ($429)

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View product citations for antibody GTX01495-11 on CiteAb

PRODUCT

Summary

The 29E.2A3 antibody reacts with human CD274 which is also known as PD-L1 or B7-H1. CD274 is a type 1 transmembrane glycoprotein and a member of the Ig superfamily. PD-L1 is a ligand for CD279 (PD-1) and plays an important role in regulating immune cell responses. CD274 is expressed on activated monocytes, macrophages, dendritic cells, and T cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD274 molecule , B7H , B7H1 , CD274 , PDCD1L1 , PDCD1LG1 , PDL1 , PD-L1

Cellular Localization

Cell membrane,Endomembrane system

Background

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Gene ID: 29126 CD274
UniProt: Q9NZQ7 CD274

Research Area

DATA IMAGES

GTX01495-11 FACS Image

FACS analysis of human PBMCs (PHA stimulation for 3 days) using GTX01495-11 PD-L1 antibody [29E.2A3] (PerCP-Cy5.5).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 1 μg (5 μl)