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PDK4 antibody, Internal

Anti-PDK4 antibody, Internal used in Western Blot (WB). GTX81596
Anti-PDK4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81596
Anti-PDK4 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81596

Cat. No. GTX81596

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
400 μl ($399)
See all PDK4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human PDK4.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase kinase 4

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PDK4 antibody, Internal used in Western Blot (WB). GTX81596

GTX81596 WB Image

WB analysis of HL-60 whole cell lysate using GTX81596 PDK4 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:1000

Anti-PDK4 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81596

GTX81596 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX81596 PDK4 antibody, Internal.

Anti-PDK4 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81596

GTX81596 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81596 PDK4 antibody, Internal.
Green : PDK4
Red : Actin filament

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399