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PER3 antibody [GT1327]

Recombinant antibody Orthogonal
Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239
Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239
Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239
Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239
Anti-PER3 antibody [GT1327] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03239

Cat. No. GTX03239

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1327

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all PER3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PER3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

period circadian regulator 3 , FASPS3 , GIG13

Cellular Localization

Cytoplasm , Nucleus

Background

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239

GTX03239 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER3 antibody [GT1327] (GTX03239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239

GTX03239 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER3 antibody [GT1327] (GTX03239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239

GTX03239 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER3 antibody [GT1327] (GTX03239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PER3 antibody [GT1327] used in Western Blot (WB). GTX03239

GTX03239 WB Image

WB analysis of various samples using GTX03239 PER3 antibody [GT1327].
Dilution : 1:1000
Loading : 25μg per lane

Anti-PER3 antibody [GT1327] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03239

GTX03239 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX03239 PER3 antibody [GT1327].
Blue : DAPI for nuclear staining
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439