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PGAM2 antibody [AT5A7]

Anti-PGAM2 antibody [AT5A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57652
Anti-PGAM2 antibody [AT5A7] used in Western Blot (WB). GTX57652

Cat. No. GTX57652

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT5A7

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PGAM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT5A7 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGAM4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate mutase 2 , GSD10 , PGAM2 , PGAMM

Background

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PGAM2 antibody [AT5A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57652

GTX57652 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57652 PGAM2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-PGAM2 antibody [AT5A7] used in Western Blot (WB). GTX57652

GTX57652 WB Image

WB analysis of various samples using GTX57652 PGAM2 antibody.
Lane 1 : Recombinant Human PGAM2 protein (50 ng)
Lane 2 : HeLa whole cell lysate (40 μg)
Lane 3 : HepG2 whole cell lysate (40 μg)
Lane 4 : 293T whole cell lysate (40 μg)
Lane 5 : Jurkat whole cell lysate (40 μg)
Lane 6 : NIH-3T3 whole cell lysate (40 μg)
Lane 7 : A549 whole cell lysate (40 μg)
Lane 8 : MCF-7 whole cell lysate (40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399