APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT25C8 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPARGC3A protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PPARG coactivator 1 alpha , LEM6 , PGC1 , PGC1(alpha) , PGC1A , PGC1alpha , PGC1v , PPARGC1 , PPARGC1A , PGC1 alpha
Cellular Localization
Nucleus,PML body,Cytoplasm
Background
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707](/templates/default/js/lightbox/images/loading.gif "Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707")
|
GTX57707 WB Image
WB analysis of various samples using GTX57707 PGC1 alpha antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : 293T whole cell lysate
Lane 5 : Mouse brain tissue lysate
Loading : 40 μg
Dilution : 1:1000
|
|
|
GTX57707 FACS Image
FACS analysis of HeLa cells using GTX57707 PGC1 alpha antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
|
REFERENCE
There are currently no references for PGC1 alpha antibody [AT25C8] (GTX57707). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PGC1 alpha antibody [AT25C8] (GTX57707). Be the first to share your experience with this product.
![Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707](/upload/website/prouct_img/small/GTX57707/GTX57707_20200204_WB_w_23061123_872.webp "Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707")
![Anti-PGC1 alpha antibody [AT25C8] used in Flow cytometry (FACS). GTX57707](/upload/website/prouct_img/small/GTX57707/GTX57707_20200204_FC_w_23061123_303.webp "Anti-PGC1 alpha antibody [AT25C8] used in Flow cytometry (FACS). GTX57707")
![Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707](/upload/website/prouct_img/normal/GTX57707/GTX57707_20200204_WB_w_23061123_872.webp "Anti-PGC1 alpha antibody [AT25C8] used in Western Blot (WB). GTX57707")
![Anti-PGC1 alpha antibody [AT25C8] used in Flow cytometry (FACS). GTX57707](/upload/website/prouct_img/normal/GTX57707/GTX57707_20200204_FC_w_23061123_303.webp "Anti-PGC1 alpha antibody [AT25C8] used in Flow cytometry (FACS). GTX57707")