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PGLS antibody

Anti-PGLS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120327
Anti-PGLS antibody used in Western Blot (WB). GTX120327
Anti-PGLS antibody used in IHC (Paraffin sections) (IHC-P). GTX120327
Anti-PGLS antibody used in IHC (Paraffin sections) (IHC-P). GTX120327

Cat. No. GTX120327

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PGLS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

SK-N-SH

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGLS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163782

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6-phosphogluconolactonase , 6PGL , HEL-S-304

Background

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.

Database

Research Area

DATA IMAGES

Anti-PGLS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120327

GTX120327 ICC/IF Image

PGLS antibody detects PGLS protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGLS protein stained by PGLS antibody (GTX120327) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PGLS antibody used in Western Blot (WB). GTX120327

GTX120327 WB Image

Sample (30 ug of whole cell lysate)
A: SK-N-SH
12% SDS PAGE
GTX120327 diluted at 1:5000

Anti-PGLS antibody used in IHC (Paraffin sections) (IHC-P). GTX120327

GTX120327 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PGLS(GTX120327) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGLS antibody used in IHC (Paraffin sections) (IHC-P). GTX120327

GTX120327 IHC-P Image

PGLS antibody detects PGLS protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
PGLS antibody (GTX120327) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Changzheng Li et al. Front Cell Dev Biol 2021; 9 : 753196 6-Phosphogluconolactonase Promotes Hepatocellular Carcinogenesis by Activating Pentose Phosphate Pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169