APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299 , mouse brain , rat brain , 293T
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952495
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1
Cellular Localization
Cytoplasm
Background
UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX109646 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:3000.
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GTX109646 WB Image
Wild-type (WT) and PGP9.5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109646 ICC/IF Image
PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX109646 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:10000.
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GTX109646 WB Image
Sample (30 ug of whole cell lysate)
A: A549
B: H1299
12% SDS PAGE
GTX109646 diluted at 1:5000
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GTX109646 IHC-P Image
PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX109646 IHC-Fr Image
PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX109646 WB Image
PGP9.5 antibody detects UCHL1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGP9.5 antibody (GTX109646) dilution: 1:5000
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GTX109646 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SEROUS OVCA xenograft, using PGP9.5(GTX109646) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109646 IHC-Fr Image
PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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