APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 5 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Spleen Tissue Slide , Human Skeletal Muscle Tissue Lysate
Product Note
This antibody only detects isoform 1 of PIG-Y.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PIG-Y.The immunogen is located within the last 50 amino acids of PIG-Y.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatidylinositol glycan anchor biosynthesis class Y , HPMRS6 , PIG-Y
Cellular Localization
Endoplasmic reticulum membrane
Background
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.
Database
DATA IMAGES
|
GTX85360 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85360 PIG-Y antibody.
Working concentration : 2.5 μg/ml
|
|
|
GTX85360 WB Image
WB analysis of human skeletal muscle tissue lysate using GTX85360 PIG-Y antibody.
Working concentration : 5 μg/ml
|
|
|
GTX85360 IHC-P Image
IHC-P analysis of human spleen tissue using GTX85360 PIG-Y antibody.
Working concentration : 20 μg/ml
|
REFERENCE
There are currently no references for PIG-Y antibody (GTX85360). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PIG-Y antibody (GTX85360). Be the first to share your experience with this product.
