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PIGK antibody [N1C2]

Anti-PIGK antibody [N1C2] used in Western Blot (WB). GTX105967
Anti-PIGK antibody [N1C2] used in Western Blot (WB). GTX105967
Anti-PIGK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105967

Cat. No. GTX105967

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PIGK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIGK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951229

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol glycan anchor biosynthesis class K , GPI8

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PIGK antibody [N1C2] used in Western Blot (WB). GTX105967

GTX105967 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105967 diluted at 1:1000

Anti-PIGK antibody [N1C2] used in Western Blot (WB). GTX105967

GTX105967 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX105967 diluted at 1:1000

Anti-PIGK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX105967

GTX105967 IHC-P Image

PIGK antibody [N1C2] detects PIGK protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PIGK antibody [N1C2] (GTX105967) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169