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PIK3AP1 antibody [7A11]

Anti-PIK3AP1 antibody [7A11] used in Flow cytometry (FACS). GTX83893
Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893
Anti-PIK3AP1 antibody [7A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83893
Anti-PIK3AP1 antibody [7A11] used in Western Blot (WB). GTX83893
Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893
Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893

Cat. No. GTX83893

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7A11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all PIK3AP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PIK3AP1 (NP_689552) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase adaptor protein 1 , BCAP

Cellular Localization

Cytoplasm,Cell membrane

Background

Involved in the activation of phosphoinositide 3-kinase (PI3K) in B-cells and in natural killer (NK) cells. Couples B-cell antigen receptor (BCR) to PI3K activation by providing a docking site for the PI3K subunit PIK3R1, which contributes to B-cell development. Seems to have a complementary role with CD19 in PI3K activation (By similarity). May be involved in the survival of mature B cells via activation of REL.

Database

Research Area

DATA IMAGES

Anti-PIK3AP1 antibody [7A11] used in Flow cytometry (FACS). GTX83893

GTX83893 FACS Image

FACS analysis of HEK293T cells transfected with either PIK3AP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83893 PIK3AP1 antibody [7A11].

Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893

GTX83893 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX83893 PIK3AP1 antibody [7A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIK3AP1 antibody [7A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83893

GTX83893 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PIK3AP1 plasmid using GTX83893 PIK3AP1 antibody [7A11].

Anti-PIK3AP1 antibody [7A11] used in Western Blot (WB). GTX83893

GTX83893 WB Image

WB analysis of HEK293T cells transfected with PIK3AP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83893 PIK3AP1 antibody [7A11].
Loading : 5 ug per lane

Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893

GTX83893 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX83893 PIK3AP1 antibody [7A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PIK3AP1 antibody [7A11] used in IHC (Paraffin sections) (IHC-P). GTX83893

GTX83893 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83893 PIK3AP1 antibody [7A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519