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PIM2 antibody [10A12]

Overexpression
Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886
Anti-PIM2 antibody [10A12] used in Western Blot (WB). GTX83886
Anti-PIM2 antibody [10A12] used in Flow cytometry (FACS). GTX83886
Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886
Anti-PIM2 antibody [10A12] used in Western Blot (WB). GTX83886
Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886

Cat. No. GTX83886

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10A12

Isotype

 IgG2a

Application

 WB, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all PIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Pim-2 proto-oncogene, serine/threonine kinase

Background

Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade | this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

Database

Research Area

DATA IMAGES

Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886

GTX83886 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83886 PIM2 antibody [10A12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PIM2 antibody [10A12] used in Western Blot (WB). GTX83886

GTX83886 WB Image

WB analysis of various cell lines using GTX83886 PIM2 antibody [10A12].
Loading : 35 ug per lane

Anti-PIM2 antibody [10A12] used in Flow cytometry (FACS). GTX83886

GTX83886 FACS Image

FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83886 PIM2 antibody [10A12].

Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886

GTX83886 IHC-P Image

IHC-P analysis of kidney tissue using GTX83886 PIM2 antibody [10A12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PIM2 antibody [10A12] used in Western Blot (WB). GTX83886

GTX83886 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83886 PIM2 antibody [10A12].
Loading : 5 ug per lane

Anti-PIM2 antibody [10A12] used in IHC (Paraffin sections) (IHC-P). GTX83886

GTX83886 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83886 PIM2 antibody [10A12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519