PIP5K1A antibody [N1N3]

Name: PIP5K1A antibody [N1N3]
Brand: GeneTex
SKU: GTX111953
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-PIP5K1A antibody [N1N3] used in Western Blot (WB). GTX111953

Anti-PIP5K1A antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111953

Anti-PIP5K1A antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111953

Cat. No. GTX111953

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PIP5K1A products

View product citations for antibody GTX111953 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PIP5K1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951250

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-4-phosphate 5-kinase type 1 alpha

Cellular Localization

Cell membrane , Endomembrane system , Golgi apparatus , Golgi stack

Database

Gene ID: 8394 PIP5K1A
UniProt: Q99755 PIP5K1A

Research Area

DATA IMAGES

GTX111953 WB Image

Wild-type (WT) and PIP5K1A knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIP5K1A antibody [N1N3] (GTX111953) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111953 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PIPK I alpha (GTX111953) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111953 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PIP5K1A(GTX111953) antibody at 1:500 dilution.

REFERENCE

Application Reference

Chu SL et al. Nat Commun 2024; 15 (1) : 1021 Phosphoglycerate kinase 1 acts as a cargo adaptor to promote EGFR transport to the lysosome.

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