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PJA1 antibody

Anti-PJA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31608
Anti-PJA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31608
Anti-PJA1 antibody used in Western Blot (WB). GTX31608

Cat. No. GTX31608

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PJA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25 - 0.5 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

Product Note

At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.The immunogen is located within amino acids 50 - 100 of PJA1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

praja ring finger ubiquitin ligase 1 , PRAJA1 , RNF70

Background

This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-PJA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31608

GTX31608 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31608 PJA1 antibody.
Working concentration : 20 μg/ml

Anti-PJA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31608

GTX31608 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31608 PJA1 antibody.
Working concentration : 5 μg/ml

Anti-PJA1 antibody used in Western Blot (WB). GTX31608

GTX31608 WB Image

WB analysis of human brain tissue lysate using GTX31608 PJA1 antibody.
Working concentration : (A) 0.25 and (B) 0.5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399