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PKA C alpha antibody [6D2.1]

Anti-PKA C alpha antibody [6D2.1] used in Dot blot (Dot). GTX80257

Cat. No. GTX80257

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D2.1

Isotype

 IgG1

Application

 WB, Dot

Reactivity

 Human
Package
100 μg ($399)
See all PKA C alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Dot Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PKACA , PPNAD4 , PRKACA , protein kinase cAMPactivated catalytic subunit alpha , PKA C alpha , protein kinase cAMP-activated catalytic subunit alpha

Cellular Localization

Cytoplasm,Cell membrane,Nucleus,Mitochondrion,Membrane,Cell projection,Cilium,Flagellum,Cytoplasmic vesicle,Secretory vesicle,Acrosome

Background

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-PKA C alpha antibody [6D2.1] used in Dot blot (Dot). GTX80257

GTX80257 Dot Image

Dot blot analysis of GST and GST-fusion proteins using GTX80257 PKA C alpha antibody [6D2.1] and GTX79895 GST tag antibody [S-tag-05]. The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1 : GST-Akt1
Lane 2 : GST-Akt2
Lane 3 : GST-Akt3
Lane 4 : GST-PKAc alpha
Lane 5 : GST-PKAc beta
Lane 6 : GST-PKAc gamma
Lane 7 : GST-MEK 1
Lane 8 : GST

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399