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PKA R2 (phospho Ser99) antibody [GT1304]

Recombinant antibody Orthogonal
Anti-PKA R2 (phospho Ser99) antibody [GT1304] used in Western Blot (WB). GTX03216
Anti-PKA R2 (phospho Ser99) antibody [GT1304] used in Western Blot (WB). GTX03216

Cat. No. GTX03216

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1304

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($419)
See all PKA R2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S99 of human PKA R2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-dependent type II regulatory subunit alpha , PKR2 , PRKAR2

Cellular Localization

Cytoplasm , Cell membrane

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKA R2 (phospho Ser99) antibody [GT1304] used in Western Blot (WB). GTX03216

GTX03216 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody [GT1304] (GTX03216) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKA R2 (phospho Ser99) antibody [GT1304] used in Western Blot (WB). GTX03216

GTX03216 WB Image

Orthogonal

WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37ºC for 1 hour using GTX03216 PKA R2 (phospho Ser99) antibody [GT1304].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419