PKA beta (catalytic subunit) (phospho Ser338) antibody

Cat. No. GTX25816

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ChIP assay
Reactivity	Human, Mouse
Package	50 μl ($399)

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Datasheet
Document

View product citations for antibody GTX25816 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ChIP assay	1:100

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

Peptide competition data indicate that this antibody cross-reacts with the PKA nu subunit (64% homologous) and partially with the alpha subunit (82% homologous).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKA catalytic b subunit that contains serine 338. The sequence is conserved in cow and pig.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cAMP-activated catalytic subunit beta , PKA C-beta , PKACB

Cellular Localization

Cytoplasm,Cell membrane,Membrane,Nucleus

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]

Database

Gene ID: 5567 PRKACB
UniProt: P22694 PRKACB

Research Area

DATA IMAGES

GTX25816 ChIP assay Image

ChIP analysis of human 5A8 J-Lat T lymphocytes culture treated with 10 μg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream and exon-1 of the EGR1 gene.
IP reaction : 1.0ul antibody / 100ul sample

GTX25816 WB Image

WB (peptide competition) analysis of 3T3-L1 cells using GTX25816 PKA beta (catalytic subunit) (phospho Ser338) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 1) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty