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PKC delta antibody [GT1232]

Recombinant antibody
Anti-PKC delta antibody [GT1232] used in Western Blot (WB). GTX02829
Anti-PKC delta antibody [GT1232] used in IHC (Paraffin sections) (IHC-P). GTX02829
Anti-PKC delta antibody [GT1232] used in Western Blot (WB). GTX02829
Anti-PKC delta antibody [GT1232] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02829
Anti-PKC delta antibody [GT1232] used in IHC (Paraffin sections) (IHC-P). GTX02829

Cat. No. GTX02829

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1232

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all PKC delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PKCdelta

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C delta , ALPS3 , CVID9 , MAY1 , PKCD , nPKC-delta

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-PKC delta antibody [GT1232] used in Western Blot (WB). GTX02829

GTX02829 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC delta antibody [GT1232] (GTX02829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC delta antibody [GT1232] used in IHC (Paraffin sections) (IHC-P). GTX02829

GTX02829 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX02829 PKC delta antibody [GT1232].
Dilution : 1:100

Anti-PKC delta antibody [GT1232] used in Western Blot (WB). GTX02829

GTX02829 WB Image

WB analysis of various samples using GTX02829 PKC delta antibody [GT1232].
Dilution : 1:1000
Loading : 25μg

Anti-PKC delta antibody [GT1232] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02829

GTX02829 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX02829 PKC delta antibody [GT1232].
Dilution : 1:100
Blue : DAPI for nuclear staining.

Anti-PKC delta antibody [GT1232] used in IHC (Paraffin sections) (IHC-P). GTX02829

GTX02829 IHC-P Image

IHC-P analysis of human thyroid cancer section using GTX02829 PKC delta antibody [GT1232].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419