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PKC mu (phospho Ser910) antibody

Orthogonal
Anti-PKC mu (phospho Ser910) antibody used in Western Blot (WB). GTX78965

Cat. No. GTX78965

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all PKC mu products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PKD/PKCμ around the phosphorylation site of serine 910 (R-V-Sp-I-L).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11174445

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PKCM,PKCMU,PKD,PRKCM,PRKD1,protein kinase D1,PKC mu

Cellular Localization

Cytoplasm,Cell membrane,Golgi apparatus,Trans-Golgi network

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-PKC mu (phospho Ser910) antibody used in Western Blot (WB). GTX78965

GTX78965 WB Image

Orthogonal

WB analysis of A431 cells, untreated or treated with EGF (200ng/ml, 10min) lysates using GTX78965 PKC mu (phospho Ser910) antibody (Lane 3 and 4) and PKC mu antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399