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PKC theta antibody

Anti-PKC theta antibody used in Western Blot (WB). GTX109690
Anti-PKC theta antibody used in Western Blot (WB). GTX109690
Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX109690
Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX109690

Cat. No. GTX109690

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PKC theta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PKC theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618690

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C theta , PRKCT , nPKC-theta

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PKC theta antibody used in Western Blot (WB). GTX109690

GTX109690 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody (GTX109690) diluted at 1:1000.

Anti-PKC theta antibody used in Western Blot (WB). GTX109690

GTX109690 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody (GTX109690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX109690

GTX109690 IHC-P Image

PKC theta antibody detects PKC theta protein at cell membrane and cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
PKC theta antibody (GTX109690) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PKC theta antibody used in IHC (Paraffin sections) (IHC-P). GTX109690

GTX109690 IHC-P Image

PKC theta antibody detects PKC theta protein at cytoplasm in rat lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
PKC theta antibody (GTX109690) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ma GS et al. Proc Natl Acad Sci U S A 2015; 112 (20) : E2602-10 Therapeutic effects of cell-permeant peptides that activate G proteins downstream of growth factors.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169