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PKC theta (phospho Thr219) antibody [HL1157]

Recombinant antibody Orthogonal
Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Western Blot (WB). GTX636461
Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636461
Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Western Blot (WB). GTX636461

Cat. No. GTX636461

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1157

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PKC theta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr219 of human PKC theta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909983

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C theta , PRKCT , nPKC-theta

Cellular Localization

Cytoplasm

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Western Blot (WB). GTX636461

GTX636461 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (GTX636461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636461

GTX636461 ICC/IF Image

Orthogonal

PKC theta (phospho Thr219) antibody [HL1157] detects PKC theta (phospho Thr219) protein by immunofluorescent analysis.
Sample: Mock and treated Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PKC theta (phospho Thr219) stained by PKC theta (phospho Thr219) antibody [HL1157] (GTX636461) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PKC theta (phospho Thr219) antibody [HL1157] used in Western Blot (WB). GTX636461

GTX636461 WB Image

Orthogonal

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Thr219) antibody [HL1157] (GTX636461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations