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PKD2 antibody [5G1]

Overexpression
Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817
Anti-PKD2 antibody [5G1] used in Western Blot (WB). GTX83817
Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817
Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817
Anti-PKD2 antibody [5G1] used in Western Blot (WB). GTX83817
Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817
Anti-PKD2 antibody [5G1] used in Flow cytometry (FACS). GTX83817
Anti-PKD2 antibody [5G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83817

Cat. No. GTX83817

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5G1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all PKD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase D2 , HSPC187 , PKD2 , nPKC-D2

Cellular Localization

Cytoplasm,Cell membrane,Nucleus

Background

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

Database

Research Area

DATA IMAGES

Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817

GTX83817 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX83817 PKD2 antibody [5G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKD2 antibody [5G1] used in Western Blot (WB). GTX83817

GTX83817 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PKD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83817 PKD2 antibody [5G1].
Loading : 5 ug per lane

Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817

GTX83817 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83817 PKD2 antibody [5G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817

GTX83817 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83817 PKD2 antibody [5G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKD2 antibody [5G1] used in Western Blot (WB). GTX83817

GTX83817 WB Image

WB analysis of various cell lines using GTX83817 PKD2 antibody [5G1].
Loading : 35 ug per lane

Anti-PKD2 antibody [5G1] used in IHC (Paraffin sections) (IHC-P). GTX83817

GTX83817 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83817 PKD2 antibody [5G1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-PKD2 antibody [5G1] used in Flow cytometry (FACS). GTX83817

GTX83817 FACS Image

FACS analysis of HEK293T cells transfected with either PKD2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83817 PKD2 antibody [5G1].

Anti-PKD2 antibody [5G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83817

GTX83817 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PKD2 plasmid using GTX83817 PKD2 antibody [5G1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519