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PKG antibody

Anti-PKG antibody used in Western Blot (WB). GTX12257

Cat. No. GTX12257

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Bovine
Package
50 μg ($399)
See all PRKG1 products

APPLICATION

Application Note

IHC: 1/800. IP: Use at a concentration of 4 μg/ml. WB: Use at a concentration of 0.5 μg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82.32 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

76 kDa. ( Note )

Positive Control

HeLa cell lysate

Product Note

This antibody recognizes a protein corresponding to the molecular mass of the PKG 1? isoform.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 14-residue synthetic peptide corresponding to residues 4-17 of human PKG 1 beta. Tryptophan was substituted for tyrosine at position 9. A terminal cysteine was added and the peptide conjugated to KLH.

Purification

Affinity purified with antigen.

Conjugation

Unconjugated

RRID

AB_369429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase cGMP-dependent 1 , AAT8 , PKG , PKG1 , PRKG1B , PRKGR1B , cGK , cGK 1 , cGK1 , cGKI , cGKI-BETA , cGKI-alpha

Cellular Localization

Cytoplasm

Background

Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-PKG antibody used in Western Blot (WB). GTX12257

GTX12257 WB Image

Western blot analysis of GTX12257
Lane 1: MW marker
Lane 2: HeLa cell lysate
Lane 3: Rat brain tissue extract
Lane 4: MDBK cell lysate.

REFERENCE

Application Reference

Lan T et al. J Ethnopharmacol 2020; 260 : 112989 Lei-gong-gen formula granule attenuates hyperlipidemia in rats via cGMP-PKG signaling pathway.
Reactivity : Rat
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399