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PKM2 antibody

Anti-PKM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50857
Anti-PKM2 antibody used in Western Blot (WB). GTX50857

Cat. No. GTX50857

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PKM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

This antibody was raised against a synthetic peptide corresponding to a region within aa 405-409 of human PKM2 (UniProt #P14618-1). Based on a BLAST result, it is specific for PKM2 and does not cross with PKM1.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.405~409(T-S-D-P-T) derived from human PKM2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate kinase M1/2 , CTHBP , HEL-S-30 , OIP3 , PK3 , PKM2 , TCB , THBP1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-PKM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50857

GTX50857 ICC/IF Image

ICC/IF analysis of methanol-fixed MEF cells using GTX50857 PKM2 antibody.

Anti-PKM2 antibody used in Western Blot (WB). GTX50857

GTX50857 WB Image

WB analysis of extracts from HepG2 and 293 cells using GTX50857 PKM2 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399