GeneTex

  • Country / Location Selection

United States (US)

PKMYT1 antibody [2B4]

Overexpression
Anti-PKMYT1 antibody [2B4] used in Western Blot (WB). GTX83875
Anti-PKMYT1 antibody [2B4] used in Flow cytometry (FACS). GTX83875
Anti-PKMYT1 antibody [2B4] used in Flow cytometry (FACS). GTX83875
Anti-PKMYT1 antibody [2B4] used in Western Blot (WB). GTX83875
Anti-PKMYT1 antibody [2B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83875

Cat. No. GTX83875

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B4

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all PKMYT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PKMYT1 (NP_004194) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase, membrane associated tyrosine/threonine 1 , MYT1 , PPP1R126

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Database

Research Area

DATA IMAGES

Anti-PKMYT1 antibody [2B4] used in Western Blot (WB). GTX83875

GTX83875 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PKMYT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83875 PKMYT1 antibody [2B4].
Loading : 5 ug per lane

Anti-PKMYT1 antibody [2B4] used in Flow cytometry (FACS). GTX83875

GTX83875 FACS Image

FACS analysis of Jurkat cells using GTX83875 PKMYT1 antibody [2B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-PKMYT1 antibody [2B4] used in Flow cytometry (FACS). GTX83875

GTX83875 FACS Image

FACS analysis of HeLa cells using GTX83875 PKMYT1 antibody [2B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-PKMYT1 antibody [2B4] used in Western Blot (WB). GTX83875

GTX83875 WB Image

WB analysis of various cell lines using GTX83875 PKMYT1 antibody [2B4].
Loading : 35 ug per lane
Dilution : 1:200

Anti-PKMYT1 antibody [2B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83875

GTX83875 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PKMYT1 plasmid using GTX83875 PKMYT1 antibody [2B4].

REFERENCE

There are currently no references for PKMYT1 antibody [2B4] (GTX83875). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PKMYT1 antibody [2B4] (GTX83875). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519