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PKR (phospho Thr451) antibody

Anti-PKR (phospho Thr451) antibody used in IHC (Paraffin sections) (IHC-P). GTX78963

Cat. No. GTX78963

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PKR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-Tp-L-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11163459

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EIF2AK1,EIF2AK2,PKR,PPP1R83,PRKR,eukaryotic translation initiation factor 2 alpha kinase 2

Cellular Localization

Cytoplasm,Nucleus,Perinuclear region

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PKR (phospho Thr451) antibody used in IHC (Paraffin sections) (IHC-P). GTX78963

GTX78963 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX78963 PKR (phospho Thr451) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399