APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 |
| 1-10 μg/ml |
| 0.5 μg/test |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659)
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP-binding cassette, sub-family D (ALD), member 3 , AI313901 , AU018866 , AW146054 , PMP68 , PMP70 , Pxmp1
Background
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX23421 WB Image
WB analysis of 25 ug of mouse lung (lane 1), mouse liver (lane 2) and HepG2 (lane 3) cell lysates using GTX23421 PMP70 antibody.
Dilution : 1:1000
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GTX23421 ICC/IF Image
ICC/IF analysis of HMVEC Cells using GTX23421 PMP70 antibody.
Red : Primary antibody
Blue : Nuclei
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GTX23421 FACS Image
FACS analysis of NIH-3T3 cells using GTX23421 PMP70 antibody compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX23421 FACS Image
FACS analysis of HepG2 cells using GTX23421 PMP70 antibody compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX23421 FACS Image
FACS analysis of 293T cells using GTX23421 PMP70 antibody compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
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GTX23421 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX23421 PMP70 antibody.
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature
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GTX23421 ICC/IF Image
ICC/IF analysis of A549 cells using GTX23421 PMP70 antibody.
Red : Primary antibody
Blue : Nuclei
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GTX23421 ICC/IF Image
ICC/IF analysis of NS-1 Cells using GTX23421 PMP70 antibody.
Red : Primary antibody
Blue : Nuclei
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GTX23421 WB Image
WB analysis of 20ug Rat Kidney (Lane 1), 20μg Rat Liver (Lane 2), 20μg Mouse Lung (Lane 3), 10μg A431 cells (Lane 4), and 10μg U2OS cells (Lane 5) per well using GTX23421 PMP70 antibody.
Dilution : 1:500
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GTX23421 ICC/IF Image
ICC/IF analysis of p19 Cells using GTX23421 PMP70 antibody.
Red : Primary antibody
Blue : Nuclei
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REFERENCE
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REVIEW
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