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PNMT antibody [N2C3]

Orthogonal
Anti-PNMT antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114098
Anti-PNMT antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114098
Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098
Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098
Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098

Cat. No. GTX114098

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PNMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HEL92.1.7,THP-1,HeLa,Caco-2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PNMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730233

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phenylethanolamine N-methyltransferase , PENT , PNMTase

Background

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PNMT antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114098

GTX114098 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PNMT(GTX114098) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PNMT antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114098

GTX114098 IHC-P Image

PNMT antibody [N2C3] detects PNMT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
PNMT stained by PNMT antibody [N2C3] (GTX114098) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098

GTX114098 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098

GTX114098 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PNMT antibody [N2C3] used in Western Blot (WB). GTX114098

GTX114098 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PNMT antibody [N2C3] (GTX114098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Saxu R et al. Int J Mol Sci 2023; 24 (24) Asymmetries of Left and Right Adrenal Glands in Neural Innervation and Glucocorticoids Production.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169