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POLH antibody

Anti-POLH antibody used in Western Blot (WB). GTX54545
Anti-POLH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54545

Cat. No. GTX54545

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all POLH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human POLH (NP_006493.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887860

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA polymerase eta , RAD30 , RAD30A , XP-V , XPV

Cellular Localization

Nucleus

Background

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-POLH antibody used in Western Blot (WB). GTX54545

GTX54545 WB Image

WB analysis of various sample lysates using GTX54545 POLH antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54545

GTX54545 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54545 POLH antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Diez M et al. 3 Biotech 2018; 8 (11) : 457 Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399