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POLR2D antibody

Anti-POLR2D antibody used in Western Blot (WB). GTX54623
Anti-POLR2D antibody used in Immunoprecipitation (IP). GTX54623
Anti-POLR2D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54623

Cat. No. GTX54623

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all POLR2D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit D , HSRBP4 , HSRPB4 , RBP4 , RPB16

Cellular Localization

Nucleus

Background

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-POLR2D antibody used in Western Blot (WB). GTX54623

GTX54623 WB Image

WB analysis of various sample lysates using GTX54623 POLR2D antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLR2D antibody used in Immunoprecipitation (IP). GTX54623

GTX54623 IP Image

IP analysis of HepG2 cell lysate using GTX54623 POLR2D antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-POLR2D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54623

GTX54623 ICC/IF Image

ICC/IF analysis of A549 cells using GTX54623 POLR2D antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399