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POLR2J antibody

Anti-POLR2J antibody used in Western Blot (WB). GTX54570
Anti-POLR2J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54570
Anti-POLR2J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54570

Cat. No. GTX54570

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all POLR2J products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA polymerase II subunit J , POLR2J1 , RPB11 , RPB11A , RPB11m , hRPB14

Cellular Localization

Nucleus

Background

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-POLR2J antibody used in Western Blot (WB). GTX54570

GTX54570 WB Image

WB analysis of various sample lysates using GTX54570 POLR2J antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-POLR2J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54570

GTX54570 ICC/IF Image

ICC/IF analysis of A549 cells using GTX54570 POLR2J antibody.

Anti-POLR2J antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54570

GTX54570 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54570 POLR2J antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399