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POR antibody [5H5]

Overexpression
Anti-POR antibody [5H5] used in Western Blot (WB). GTX83859
Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859
Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859
Anti-POR antibody [5H5] used in Western Blot (WB). GTX83859
Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859
Anti-POR antibody [5H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83859

Cat. No. GTX83859

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all Cytochrome P450 Reductase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50
IHC-P 1:50
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human POR (NP_000932) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome p450 oxidoreductase , CPR , CYPOR , P450R

Cellular Localization

Endoplasmic reticulum membrane

Background

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Database

Research Area

DATA IMAGES

Anti-POR antibody [5H5] used in Western Blot (WB). GTX83859

GTX83859 WB Image

Overexpression

WB analysis of HEK293T cells transfected with POR plasmid (Right) or empty vector (Left) for 48 hrs using GTX83859 POR antibody [5H5].
Loading : 5 ug per lane

Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859

GTX83859 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX83859 POR antibody [5H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859

GTX83859 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83859 POR antibody [5H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-POR antibody [5H5] used in Western Blot (WB). GTX83859

GTX83859 WB Image

WB analysis of various cell lines using GTX83859 POR antibody [5H5].
Loading : 35 ug per lane
Dilution : 1:200

Anti-POR antibody [5H5] used in IHC (Paraffin sections) (IHC-P). GTX83859

GTX83859 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX83859 POR antibody [5H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-POR antibody [5H5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83859

GTX83859 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83859 POR antibody [5H5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519